There will be a $290 annual cost per license. Additionally, FlowJo v11 will only be compatible with the FlowJo Portal system.Ĭost for joining the Site License: We are switching to the new portal system in August 2022. The FlowJo Portal site license will allow users to analyze their data from anywhere as long as they have access to a computer with FlowJo and internet. The 4-computer flexibility is not meant to replace 4 licenses as they cannot run the software simultaneously. Some information and benefits about joining the Site License: This license will allow you to have a username and password that can be used on up to 4 computers throughout the year. Finally, there will be more built-in plugins for data dimension reduction (ie tSNE & UMAP) as well as clustering algorithms (ie XShift, FlowSOM, FlowMeans and Phenograph) making it easier to explore and analyze high-dimensional data. There will also be increased capacity for statistical analysis including new statistical graphing features. Additionally, there will be a new quality control feature which ensure users are looking at high quality data. FlowJo 11 will be faster, and it will be easier to compare multiple samples at one time. Some things you can look forward to in FlowJo v11: FlowJo Version 11 will have multiple upgraded features. This site license will offer you the ability to always have the latest version of FlowJo. The UTMB Flow Cytometry Core now has a FlowJo Portal site license. Unlike previous upgrades, FlowJo 11 will no longer be backwards compatible with the dongle system. We’ll just need one of your data files so we can help you set the preferences accordingly.FlowJo will soon release an updated version of FlowJo (v11). If your data is not scaled properly or you still have trouble, please contact us (techsupport )! We can easily assist you in setting the preferences based on your data. Linear/logarithmic/biexponential default scaling, scale range, and auto-transform can all be set in these cytometer preferences!įor information on getting FlowJo to match the display of acquisition software, choose your vendor below:Īll of these documents are written based on our understanding of the default display of the data on the respective instrument. Each preference set can be adjusted based on how you wish to display the data. To learn more about setting up preferences for the display scaling and transformation of the data from your cytometer, click on the Cytometers button in Preferences. For specific information on data scaling visit here. Since FlowJo must scale the data identically to what you see on the machine, we have implemented a great new feature in v10 of FlowJo that will create a specific preference set based on the cytometer on which the data was acquired when loaded into FlowJo. However, instruments like the Beckman Coulter Gallios, Life Technologies Attune, BD Accuri C6, and MoFlo Astrios will scale data from 6 to 10 decades. Many people are used to visualizing data in 4 or 5 decades (a scale of 2^10 or 2^18). Instrument scaling range can vary from 2^18 on FACSDiva equipped instruments to 2^32 on MoFlo Astrios. The FCS3.0 standard provides for up to 32 bits of data, or a range in the scale of 2^32. FlowJo can read and analyze FCS files from any cytometer.Įach cytometer will scale your data slightly different during acquisition. As you probably know, FCS (Flow Cytometry Standard) files are created by flow cytometers.
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